The comparative survey of phenotypic methods and the 16S rRNA gene sequencing method for detecting genus and species of non-fermented gram-negative bacteria isolated from blood samples in Isfahan, Iran

Introduction: This study compared phenotypic methods and the 16S rRNA gene sequencing method to identify genus species of non-fermenting gram-negative bacilli isolated from blood culture samples. Objectives: The aim this was evaluate effectiveness using in detecting non-fermented bacteria Materials Methods: A cross-sectional conducted April 2019 2020, including all patients who required sterile at AL Zahra Kashani hospitals. Specimens were cultured BACTEC subjected standard methods. Subsequently, performed detect Bacillus level. comparative evaluation then conducted. Results: identified 30 bacteria. observed that 83.3% diagnoses Acinetobacter baumannii, while approach 86.7% as Acinetobacter. Conclusion: results indicate a significant difference between (NFGNB).